FIG. 5.

Immunofluorescence analysis. Vero cells were infected with wild-type virus (KOS) (top row), UL15-null virus (hr81-1) (middle row), or the UL15 mutant virus Q205E (bottom row) at an MOI of 10 PFU/cell and fixed for immunofluorescence at 8 h postinfection. Infected cells were stained with anti-ICP8 monoclonal antibody (39S) (left panels, green) and anti-UL15 polyclonal antibody (αAS9) (middle panels, red). The right-hand column labeled Merge shows the merged images. The color was adjusted on the hr81-1 panels to demonstrate the lack of UL15 in these cells.