TABLE 2.
MHV-68 M2 and M3 expression in spleen at day 14 p.i.a
| Cellsb | M2 | M3 |
|---|---|---|
| B cells (total) | 3/3 | 3/3 |
| Resting B cells | 3/5 | 4/5 |
| Activated B cells | 4/5 | 5/5 |
| Mφs | 0/3 | 3/3 |
| DCs | 0/4 | 4/4 |
a
Data represent positive RT-PCR results per number of individual sorts analyzed for each MHV-68 ORF; β-actin was equivalently detected in all samples. Mφ, macrophage; DC, dendritic cell.
b
Cells sorted as B220+ CD11b− CD11c− (total B cells; mean purity, 99.64%), B220+ peanut agglutinin (PNA)low (resting B cells; mean purity, 98.15%), B220+ PNAhigh (activated B cells; mean purity, 98.64%), B220− CD11b+ CD11c− (Macrophages; mean purity, 96.34%), and B220− CD11b+/− CD11c+ (dendritic cells; mean purity, 92.86%).