FIG. 6.

Creation and analysis of suppressors of Super M. (A) The WT numbers of positively and negatively charged residues were restored in the Super M domain by basic-to-acidic substitutions at positions 6 and 18 or at positions 13 and 18. (B) Analysis of the budding efficiencies of the charge-balanced mutants was performed with proviral vectors as for Fig. 2A. (C) The localization patterns of ΔNC-GFP and Gag-GFP proteins containing charge-balanced M domains in untreated or LMB-treated cells were examined as for Fig. 5B. (D) The genomic RNA content of extracellular virus was determined by using proviral vectors as for Fig. 4A. (E) Infectivity assays were performed at 1 and 2 weeks postinfection as for Fig. 2D. For the results shown in panels B, D, and E, the data are the averages of at least three experiments and the error bars represent 1 standard deviation from the mean.