FIG. 2.
Effect of cholesterol depletion from virions on HSV entry. HSV-1 (KOS/tk12) particles (2 × 106 PFU) were treated with various amounts of MβCD for 30 min at room temperature. MβCD was then diluted to a final concentration of 0.1 mM or less, a concentration that has no effect on cells, and virus was allowed to infect Vero cells (multiplicity of infection of 10). β-Galactosidase activity at 6 h postinfection was determined as described for Fig. 1. The results presented are representative of two independent experiments done in triplicate, with standard deviations.