FIG. 5.

Effect of cholesterol depletion from cells on the course of HSV entry. Vero cells were infected with HSV-1 (KOS/tk12). At various times following infection (indicated on the abscissa), cells were treated for 30 min with MβCD. The drug was washed out, and medium or cholesterol (chol) was added. After 30 min, the cholesterol was removed, and infection was allowed to proceed. β-Galactosidase activity at 6 h postinfection was determined. The results shown are representative of three independent experiments, and 100% entry represents the β-galactosidase activity at 6 h in the absence of MβCD.