Association of HSV receptors with detergent-insoluble glycolipid complexes. 3E5 cells (A), C10 cells (B), and control parental B78-H1 cells were fractionated into detergent-soluble (DSM) and insoluble (DIG) membrane fractions with either Triton X-100 (TX-100) or Brij-96. The distribution of the two HSV receptors was analyzed by Western blotting as described for Fig. 6. At the top are indicated the receptor expressed (control, HVEM, or nectin-1), the detergent used (Triton X-100 or Brij-96), and the fraction, DSM (M) or DIG (D). Sizes are shown on the left (in kilodaltons).