FIG. 9.

Association of HSV soluble glycoproteins with detergent-insoluble glycolipid complexes. (A) Soluble gB, (B) gC, or (C) gD (0.1 μM each) was bound to C10 cells at 4°C, and the temperature was shifted at 37°C for various times (in minutes), as indicated at the top of the panel. Cells were extracted in 1% Brij-96, and the distribution of each glycoprotein into DSM and DIG was analyzed as described for Fig. 8. For each experiment, the control (c) without glycoproteins (A and B) or on parental nectin-1-negative B78-H1 cells (C) is shown. Panel C also shows the distribution of nectin-1. Sizes are shown on the left (in kilodaltons).