FIG. 3.

Rotavirus activating effects on BBM Cl⁻/H⁺ symporter as a function of time after infection. The initial Cl⁻ entry rates were determined with 15 mM cis ³⁶Cl. The extra- and intravesicular spaces contained a 20 mM HEPES-40 mM citric acid-100 mM Tris gluconate buffer adjusted with Tris base to give an initial pH_o/pH_i gradient of 5.0/7.5. Results are expressed in nanomoles · milligrams of protein⁻¹ · second⁻¹ ± SD, with n = 18 to 72 determinations per point obtained from three to seven different membrane preparations. Identical letters indicate results found to be statistically indistinguishable according to a global F test (P < 0.01).