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Combined influence of NC and MA mutations on RNA packaging. RNA packaging efficiencies were determined by the ratio of viral RNA to the amount of Gag from VLPs (see Materials and Methods for details). The RNA packaging efficiency with wild-type BLV was set at 100, and the values for the mutants (± standard deviation) are relative to that for the wild type (WT). Mutants were tested in parallel with the parental construct, and these experiments were done in triplicate.
