TABLE 1.
Experiments with fusion proteins containing terminally truncated fragments of the RUB and SIN C genesa
| Cassette | Replication (GFP/RNA) | Complementation
|
|
|---|---|---|---|
| cis (GFP/RNA) | trans (GFP) | ||
| RUB C | |||
| 3′ deletions | |||
| C(1-277)-GFP/CAT | +/+ | +/+ | + |
| C(1-199)-GFP/CAT | +/+ | −/− | + |
| C(1-151)-GFP/CAT | +/+ | −/− | + |
| C(1-118)-GFP/CAT | +/+ | −/− | + |
| C(1-88)-GFP/CAT | +/+ | −/− | + |
| C(1-58)-GFP/CAT | +/+ | −/− | + |
| C(1-50)-GFP/CAT | +/+ | −/− | + |
| C(1-44)-GFP/CAT | +/+ | −/− | + |
| C(1-31)-GFP/CAT | +/+ | −/− | + |
| C(1-18)-GFP/CAT | +/+ | −/− | − |
| C(1-8)-GFP/CAT | +/+ | −/− | − |
| 5′ deletions | |||
| C(9-88)-GFP/CAT | +/+ | −/− | − |
| C(9-300)-GFP/CAT | +/+ | −/− | − |
| C(18-88)-GFP/CAT | +/+ | −/− | − |
| C(18-300)-GFP/CAT | +/+ | −/− | − |
| C(19-300)-GFP/CAT | +/+ | −/− | − |
| C(58-300)-GFP/CAT | +/+ | −/− | − |
| C(88-300)-GFP/CAT | +/+ | −/− | − |
| C(118-300)-GFP/CAT | +/+ | −/− | − |
| C(151-300)-GFP/CAT | +/+ | −/− | − |
| C(199-300)-GFP/CAT | +/+ | −/− | − |
| C(277-300)-GFP/CAT | +/+ | −/− | − |
| SIN C | |||
| C(1-264)-GFP/CAT | +/+ | −/− | − |
| C(1-220)-GFP/CAT | +/+ | −/− | − |
| C(1-160)-GFP/CAT | +/+ | −/− | − |
| C(1-100)-GFP/CAT | +/+ | −/− | − |
| C(1-40)-GFP/CAT | +/+ | −/− | − |
a
The designation for each construct gives the amino acid residues retained in the fragment expressed by that construct: the complete RUB C gene is C(1-300), while the complete SIN C gene is C(1-264). The replication results represent the GFP cassette in RUBrep, and the complementation results represent the GFP cassette in RUBrep-ΔNotI (cis) and the CAT cassette in RUBrep + RUBrep/GFP-ΔNotI (trans).