TABLE 2.

GFP and CAT activity in Vero cells

Transfection	Activity
	GFPa	CAT (cpm)b
Mock	−	374
RUBrep/CAT-ΔNotI	−	364
C(1-8)-CAT-ΔNotI	−	350
C(1-18)-CAT-ΔNotI	−	338
C(1-31)-CAT-ΔNotI	−	328
C(1-44)-CAT-ΔNotI	−	319
C(1-50)-CAT-ΔNotI	−	352
C(1-58)-CAT-ΔNotI	−	285
C(1-88)-CAT-ΔNotI	−	16,185
C(1-151)-CAT-ΔNotI	−	15,243
C(1-199)-CAT-ΔNotI	−	287
C(1-277)-CAT-ΔNotI	+	4,689
C-CAT-ΔNotI	+	2,324
C-E2-CAT-ΔNotI	+	2,451

^aVero cells were transfected with transcripts from GFP-ΔNotI. At 4 days posttransfection, GFP expression was monitored by direct examination of the transfected monolayer with a Zeiss Axioplan microscope with epifluorescence capability.

^bVero cells were transfected with transcripts from replicon-CAT-ΔNotI. At 4 days posttransfection, cells were lysed and assayed for CAT activity. CAT activity is given as the amount of [³H]acetyl coenzyme A partitioning into the aqueous phase following a reaction in the presence of chloramphenicol (32). The activity given is the average from three independent experiments. The low activity expressed by C(1-199)-CAT-ΔNotI is likely due to formation of aggregates by this fusion protein.