FIG. 2.
Torovirus genome organization and schematic outline of the strategies used for RT-PCR amplification of the genes for the structural proteins. The upper panel shows a schematic representation of the genome. Boxes represent the genes for the polymerase (ORF1a and ORF1b), the spike protein (S), the membrane protein (M), the hemagglutinin-esterase (HE), and the nucleocapsid protein (N). Open arrows indicate intergenic regions and transcription-regulating sequences. Also indicated are the cap structure (black dot) and the poly(A) tail (An). The lower panel shows a schematic outline of the RT-PCR assays employed to amplify S, M, HE, and N sequences. The orientations and positions of the oligonucleotides on the torovirus genome are shown.