Figure 7.

A rostral-caudal hermaphrodite at NF stage 66, treated with 0.1 ppb atrazine. Abbreviations: K, kidney, O, ovary, T, testis. (A) The entire dissected, Bouin’s-fixed kidney–interrenal–gonadal complex. (B,C) Transverse cross-sections (8 μm) of the animal’s right gonad stained in Mallory’s trichrome stain. The sections were taken from the areas shown by the white arrowheads: right rostral testis in cross-section (B) and right caudal ovary (C). (D–G) Sagittal sections (8 μm) taken from the animal’s left gonad from the areas indicated by the white arrows: sagittal sections of the left rostral testis (D,E) and through the caudal ovary ( F,G). Note absence of pigment in the ovary. Scale bar: (A) 0.1 mm; (B–C) 10 μm; (D–G) 50 μm.