FIG. 4.

Protein gel analysis of influenza A/WSN and recombinant WSN-BHA/ACT-ATM (P6) viruses. Proteins of purified viruses, either untreated (−) or treated with PNGase F (+), were separated by SDS-PAGE on a 12% gel and stained with Coomassie brilliant blue. Positions of untreated (−) and treated (+) proteins are indicated. The band of added PNGase F is shown to the right by an arrow. The positions of the deglycosylated influenza A/WSN HA₁ (Flu A HA₁) and WSN-BHA/ACT-ATM (Flu B HA₁) viruses are indicated by arrows on the right. HA₀, HA precursor protein; HA₂, HA₂ HA subunit.