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Content of influenza B virus NA in purified chimeric viruses as determined by ELISA. ELISA plates were coated with 50 μl of serial twofold dilutions of recombinant purified WSN-BNA (▴), WSN-BNA/A65 (▵), WSN-BNA/ACT (▪), WSN-BNA/ACT-ATM (□), and wild-type B/Yamagata viruses (⋄). The starting concentration was 5 μg/ml. Plates were incubated with an influenza B virus NA-specific monoclonal antibody (9A6) and developed with a secondary horseradish peroxidase-conjugated anti-mouse IgG.
