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High frequencies of TNF-α- and IL-2-positive CD4+ T cells in HAM/TSP patients after 6 h of in vitro culture with HTLV-1 peptides. Data on TBO, a patient with HAM/TSP, are shown. Cells were gated on the CD4hi population. The total number of events collected was 200,000 for the peptide-stimulated culture and 100,000 for the control (no peptides).
