Figure 3.

Cloning and alignment of the deduced amino acid sequences of chicken GC1 with human, bovine, mouse, and rat GC1. (A) Schematic showing the 21 GC1 cDNA clones obtained by cDNA library screening, RT-PCR, and 5′ RACE. The 988-bp Southern and 500-bp Northern GC1 probes are shown above the GC1 domain map. The numbers used to identify the clones correspond to primers whose sequences appear in the Methods section. sp, signal peptide; ext, extracellular domain; m, membrane-spanning domain; kin, kinase-like domain; cat, catalytic domain. (B) Alignment of GC1 extending from the membrane-spanning domain to the kinase-like domain. The 29-aa peptide present in approximately one-half of the chicken GC1 transcripts (X7A) is shown. Residues printed in white are identical or represent conservative substitutions across all five sequences (L = I = V = M; E = D; S = T = A; r = K). Residues printed black on gray are identical or conserved substitutions in four of the five sequences. Nonconserved sequences are printed in black on white.