FIG. 2.

Expression of PRV gC and gD in ORFV recombinant-infected cells. Vero cells were infected (MOI of 10) with D1701-VrVgC (lanes 1), D1701-VrVgD (lanes 2), D1701-VrV (lanes 3), and PRV Begonia (lanes 5) or mock infected (lanes 4). For Northern blot hybridization using radioactively labeled probes specific for PRV gC (A), PRV gD (B) and the ORFV early gene ANK3 (C), total RNA was isolated from CH-treated Vero cells. The sizes of the respective specific transcripts are indicated in kilobase pairs to the left. Cell lysates were obtained 24 h p.i., and Western blot analysis was performed with the gC-specific MAb A18b (D), the gD-specific rabbit serum 016/00 (E), or the ORFV 39K-specific MAb 4D9 (F). The apparent molecular mass of the detected proteins is indicated in kilodaltons.