Fig. 5.

Restoration of motor and cognitive function in ASMKO mice. Analysis of motor function by accelerating (A) and rocking (B) rotarods, and cognitive function by the Barnes maze (C). The combination group performed significantly better than untreated ASMKO and AAV2 brain- and AAV8 systemic-alone groups on both rotarod tests throughout the entire time course (P < 0.001). However, the combination group was not completely asymptomatic when compared to untreated wild-type controls (A and B). In the Barnes maze, the combination group performed at wild-type levels throughout the time course of the study, which did not occur in the singly treated groups (C). ∗, P < 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001. In addition, comparisons between the AAV2 brain-alone group and the combination group, and between the combination group and untreated wild-type mice, are shown in brackets. Open square, combination group; open circle, AAV8 systemic-alone group; filled square, AAV2 brain-alone group; filled circle, untreated ASMKO mice; filled triangle, untreated wild type.