Fig. 1.
Cellular localization of LBR1–238GFP transiently expressed in Sf9 cells. (a) Confocal microscopy images of LBR1–238GFP. A single z-section is shown. Calnexin labeling is red, LBR1–238GFP is green, and lamin Dm0 is white. For ease of viewing, lamin Dm0 is recolored in blue in the merge image. (b and c) Sf9 cells transiently expressing LBR1-238GFP were fixed, thin sectioned, and analyzed by using GFP antibody and 25 nM gold-conjugated secondary antibody. The membranes are outlined, ONM and INM are labeled, and arrows point to the location of LBR1–328GFP. (d) FRAP was used to determine recovery kinetics of LBR1–238GFP in CHOK1 and Sf9 cells. (Upper) A graphical recovery curve of LBR1–138GFP in Sf9 cells. (Lower) Calculated diffusion constants for the complete analyses. Total sample size is listed in parentheses.