FIG. 6.

KSHV reactivation and K-bZIP expression prolong G₀/G₁ phase. (A) Cell cycle analysis of TPA-treated K-bZIP-BCBL-1 cells. Cell cycle analysis after induction by TPA was carried out with flow cytometry. Cell cycle analysis was performed with ModFit LT. The percentage of cells in G₀/G₁ phase is indicated. (B) Upregulation of p21 and p27 in K-bZIP-BCBL-1 cells after induction. Protein extract (100 μg/lane) from each time point (in hours) was loaded. Protein expression levels of p21, p27, and total K-bZIP in HA-K-bZIP-BCBL-1 cells were measured by immunoblotting. Actin served as an internal control for the amount of protein on the membrane. α, anti.