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. 2007 Mar 26;403(Pt 2):243–250. doi: 10.1042/BJ20061123

Figure 3. Time course of CG7 action.

Figure 3

(A) CHO/IR cells pre-incubated for 10 min with CG7 or vehicle were stimulated with 1 nM insulin. At various times the cells were solubilized, and ELISAs for IRβ auto-phosphorylation were performed as described in the Experimental section. Values are means±S.D. for three experiments. *, P<0.05; **, P<0.005. (B) CHO/IR cells were incubated with 1 nM insulin, and 10 μg/ml CG7 was added after 10 min. Cells were lysed after various incubation times and ELISAs for IRβ auto-phosphorylation were performed. Values are means±S.D. for three experiments.