Figure 6. Demonstration of rPrP^10OR and rPrP^C aggregates by SDS/PAGE.

rPrP^C (5 μM) was mixed with 0.1, 0.5, 1 or 2 μM rPrP^10OR in 50 mM sodium acetate and 150 mM NaCl (pH 4.0), followed by the addition of 0.5 M GdmCl at 37 °C. After 90 min, the aggregates were collected by centrifugation and washed as described in the Experimental section. The pellets were dissolved by heating in 1× SDS loading buffer and the samples were separated by SDS/PAGE under reducing conditions (top panel). The amount of rPrP^C and rPrP^10OR in each precipitate was quantified by densitometric analysis (lanes 2–8 and 11) (bottom panel). The experiment was performed at least three times.