TABLE 2.
Colony formation efficiency of total cellular RNAa
| Cell | Viral RNA (ng/10 μg) | No. of colonies in transfected cells
|
|||||
|---|---|---|---|---|---|---|---|
| Huh7
|
HeLa
|
Hepa1-6
|
|||||
| Mean (SD) | Colonies/ng of viral RNA | Mean (SD) | Colonies/ng of viral RNA | Mean (SD) | Colonies/ng of viral RNA | ||
| GS4.1 | 5 | 834 (64) | 166 | 22 (4) | 4 | 0 | <1 |
| SL1 | 5 | 100 (53) | 20 | 803 (81) | 160 | 1.3 (1.5) | <1 |
| MH1 | 0.5 | 20 (2) | 40 | 66 (9) | 132 | 1.7 (0.6) | 3 |
a
Results from three independent transfection experiments. Total RNA was extracted from GS4.1, SL1, and MH1 cells at passages 21, 26, and 4, respectively.