TABLE 4.
Colony formation efficiency of mutant replicons in Huh7 cells
| Vector | Conserved mutation(s) | Mean (SD) CFE/μg of RNAg | NS protein(s) affected |
|---|---|---|---|
| I377/NS3-3′ | NAh | 2.3 (1.5) | NA |
| pZS10 | A1a | 3.3 (3.5) | NS3 |
| pZS1 | C1b | 1.4 × 103 (3.9 × 102) | NS5A |
| pZS20 | Bc | 1.7 (0.6) | NS4AB |
| pZS11 | A1 + C1 | 2.4 × 105 (7 × 104) | NS3, NS5A |
| pZS2 | B + C1 | 7.8 × 104 (1.7 × 104) | NS4AB, NS5A |
| pZS12 | A1 + B | 0.3 (0.6) | NS3, NS4AB |
| pZS5 | A1 + B + C1 | 15 | NS3, NS4AB, NS5A |
| pZS4 | B + C1 + A2d | 165 (21) | NS3, NS4AB, NS5A |
| pZS8 | A2 + B | 0 | NS3, NS4AB |
| pZS6 | A2 + B + C2e | 8 (4) | NS3, NS4AB, NS5A |
| pZS15 | C3f | 491 (183) | NS5A |
| pZS25 | B + C3 | 1.7 × 104 (2.6 × 103) | NS4AB, NS5A |
| pZS45 | A2 + B + C3 | 19 (2) | NS3, NS4AB, NS5A |
a
A1, mutation E1202G.
b
C1, mutations S2204I and D2254E.
c
B, mutations L1701F, Q1720R, Q1727H, and V1749A.
d
A2, mutations E1202G, S1128A, and S1323P.
e
C2, mutations S2204I, D2254E, R2290I, and I2324R.
f
C3, all the mutations of C2 plus the deletion 2371-2413.
g
CFE, colony formation efficiency. Values are derived from three independent transfections of each replicon RNA.
h
NA, not applicable.