Figure 1. Schematic representations of mouse Tbc1d1 and AS160.

The amino acid sequences used are NCBI gi 37538012 for mouse Tbc1d1 and NCBI gi 67462068 for mouse AS160. Tbc1d1 and AS160 are proteins of 1255 and 1307 amino acids respectively. The two PTB domains and the GAP domains are those predicted by analysis with the Pfam program (http://pfam.wustl.edu). The wavy line at the border of a domain indicates that the domain alignment is incomplete at that end of the domain. SV designates the section deleted from the shorter slice variant. CM designates the calmodulin-binding motif. The sites of insulin-stimulated phosphorylation of AS160, most likely by the protein kinase Akt, are numbered, as are the two such sites conserved in Tbc1d1. The numbers in parentheses are the numbers of the same Akt sites in the human versions of Tbc1d1 (NCBI gi 54887445) and AS160 (NCBI gi 114688046). Thr⁵⁹⁰ (Thr⁵⁹⁶ in humans) of Tbc1d1 corresponds to Thr⁶⁴⁹ (Thr⁶⁴² in humans) of AS160; Ser⁵⁰¹ (Ser⁵⁰⁷ in humans) of Tbc1d1 corresponds to Ser⁵⁷⁷ (Ser⁵⁷⁰ in humans) of AS160.