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. 2007 Mar 26;403(Pt 2):267–274. doi: 10.1042/BJ20061707

Table 2. Specific activity and kinetic constants for OaGST and the S11A mutant with CDNB as the second substrate.

Results are means±S.D. for at least three independent determinations.

GSH CDNB
Enzyme Specific activity (μmol·min−1·mg−1) Km (mM) kcat (s−1) kcat/Km (mM−1·s−1) Km (mM) kcat (s−1) kcat/Km (mM−1·s−1) pKa (CDNB)
OaGST 5.9±0.43 0.133±0.01 2.36±0.12 17.78 3.094±0.19 16.5±0.82 5.33 5.96±0.17
S11A 2.2±0.15 1.917±0.12 9.23±0.56 4.81 1.187±0.08 14.1±0.49 11.87 5.84±0.22