Table 2. Specific activity and kinetic constants for OaGST and the S11A mutant with CDNB as the second substrate.
Results are means±S.D. for at least three independent determinations.
| GSH | CDNB | |||||||
|---|---|---|---|---|---|---|---|---|
| Enzyme | Specific activity (μmol·min−1·mg−1) | Km (mM) | kcat (s−1) | kcat/Km (mM−1·s−1) | Km (mM) | kcat (s−1) | kcat/Km (mM−1·s−1) | pKa (CDNB) |
| OaGST | 5.9±0.43 | 0.133±0.01 | 2.36±0.12 | 17.78 | 3.094±0.19 | 16.5±0.82 | 5.33 | 5.96±0.17 |
| S11A | 2.2±0.15 | 1.917±0.12 | 9.23±0.56 | 4.81 | 1.187±0.08 | 14.1±0.49 | 11.87 | 5.84±0.22 |