Figure 4. Temperature-dependent cellular fluorescence profiles for R8–Alexa Fluor® 488 peptides and Alexa Fluor® 488–Tf.

KG1a cells were incubated at various temperatures between 4 and 37 °C for 1 h with 2 μM L- or D-R8–Alexa Fluor® 488 or 100 nM Alexa Fluor® 488–Tf before washing and analysis by flow cytometry. Unfilled peaks represent untreated cells.