Figure 7. Effects of cholesterol depletion on cellular distribution of R8–Alexa Fluor® 488 peptides and Alexa Fluor® 488–Tf.

KG1a cells were pre-incubated in the absence (Control) or presence (MβCD) of 5 mM MβCD for 30 min at 37 °C before washing and incubation with 2 μM L- or D-R8–Alexa Fluor® 488 or 100 nM Alexa Fluor® 488–Tf for 1 h at 37 °C. The cells were then washed and analysed by fluorescence microscopy. Scale bars, 10 μm.