Table 1. Analysis of fluorescence peaks obtained from incubating cells with increasing concentrations of R8–Alexa Fluor® 488 peptides.

KG1a cells were incubated with 0.25–5.0 μM L- or D-R8–Alexa Fluor® 488 for 1 h at 4 °C. The cells were then washed and either immediately analysed by flow cytometry (PBS) or treated further with trypsin and heparin before analysis (trypsin/heparin). Two peaks of fluorescence were observed and designated Low and High (Figure 5). Shown are the geometric mean values for the Low and High peaks for all depicted concentrations. Results are means±S.D.for two individual experiments performed in duplicate. Statistical analysis for comparing the geometric means of untreated compared with treated fluorescence cells was performed using Student's t test. *P<0.05; decreased relative to PBS control.

(a) L-R8
	Low		High
Peptide concentration (μM)	PBS	Trypsin/heparin	PBS	Trypsin/heparin
0.25	2.76±0.14	2.78±0.45	17.20±0.45	16.10±0.26*
0.50	3.12±0.13	2.94±0.12	39.99±3.27	37.87±2.35
1.00	3.89±0.23	3.16±0.22*	98.88±4.60	93.64±2.74
2.00	4.78±0.28	3.51±0.19*	175.14±10.61	165.90±14.05
5.00	9.44±1.21	4.69±0.47*	357.73±62.42	338.55±46.47
(b) D-R8
	Low		High
Peptide concentration (μM)	PBS	Trypsin/heparin	PBS	Trypsin/heparin
0.25	3.18±0.12	2.94±0.19	15.73±1.27	12.46±0.57*
0.50	4.09±0.18	3.07±0.05*	28.71±5.20	30.77±4.56
1.00	4.80±0.51	3.17±0.17*	83.14±19.10	76.78±17.98
2.00	6.07±0.17	3.82±0.28*	147.86±16.75	155.025±13.61
5.00	11.83±1.69	6.18±1.05*	430.48±63.94	521.81±105.11