TABLE 1.

Locations and sequences of siRNA targets

siRNA no.a	Location of target (5′-3′)b	Sequence of target (5′-3′)c
1	1530-1510	AAGAAAGAAGUUAGAGGAACU
2	1472-1452	AAGAUAGAGGACGAAAAUCCC
3	1374-1354	AACGGACCAGAUGGAGGUAGA
4	1287-1267	AAGGAAGGCCCUCGAGAACAA
5	1271-1251	AACAAGAAGAAGCAGCUAUCG
6	1241-1221	AAGAACCUCAGCAAGGAGGAA
7	1219-1199	AAGAGGAACUCAGGAGGUUGA
8	1292-1272	AAGACGAGAGAAGGGAAAGAA
9	1029-1009	AAACCAGGGAUUUCCAUAGGA
10	67-87	AAAGAGCAUUGGAACGUCGGA
11	248-268	AAGGGUUGAGUAGCACUCAGA
12	399-419	AAGCGAGGAGGAAAGCAAAGA
13	486-506	AACUCGACUUAUCGUCCCCAU
14	76-56	AAUGCUCUUUACCGUGACAUC
15	339-319	AAGCGCCUCUUGUUCGCUGAA
16	495-475	AAGUCGAGUUCCCCGGGAUAA

^aThe numbers of the siRNA species are as in Fig. 2.

^bThe positions are presented using the HDV genome numbering of Kuo et al. (15). The numbers go “up” in the 5′ to 3′ direction of the genomic strand but “down” in the 5′ to 3′ direction of the antigenomic strand.

^cEach siRNA was assembled as a short double-stranded RNA. The two strands were transcribed and assembled using a Silencer siRNA construction kit (Ambion) with chemically synthesized DNAs as a template. The 21-nt sequences shown, all of which begin with AA, represent the target on the HDV RNA, be it genomic or antigenomic, that is targeted by the double-stranded siRNA. Each siRNA has 19 nt of double-strandedness with two unpaired nucleotides, UU, at the 3′ end. It has 21 nt complementary to the target sequence and only 19 nt complementary to the opposite strand.