FIG. 4.

Confocal microscopy of cells expressing Gag-GFP and wt or N-terminally truncated Tsg101-Myc. Shown are confocal images of fixed COS-1 cells transfected with Gag-GFP alone (A), Gag-GFP and wt Tsg101-Myc (B), or Gag-GFP and N-terminally truncated Tsg101-Myc (fragment E) (C). Serial z sections from the adherent cell surface through the nucleus are shown for the region indicated by the dashed white square. In panel B, view 1 corresponds to the upper white square and view 2 corresponds to the lower white square. Green, Gag; red, Tsg101-Myc; blue, nuclei stained with DAPI.