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. 2002 Sep 15;16(18):2379–2389. doi: 10.1101/gad.1020702

Figure 1.

Figure 1

Western blot of nuclear extracts from S2 cells, 1–5-h embryos, and first-instar larvae. The anti-serum used is indicated under each panel. S2 cells and 1–5-h embryos express both full-length and an N-terminally truncated form of STAT92E (lanes 2–5). In first-instar (1*) larvae that are stat92E−/− null mutants, no STAT92E is detected as compared with wild-type larvae, showing that the faster-migrating band is not caused by cross-reaction with a protein other than STAT92E (lane 6). Wild-type first-instar larvae predominantly express the N-terminally truncated form of STAT92E (lane 7). Flag-tagged recombinant STAT92E was transfected into S2 cells, and the extract was run simultaneously but blotted with Flag antibody separately, to indicate the position of full-length STAT92E (lane 1).