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. 2002 Sep 15;16(18):2379–2389. doi: 10.1101/gad.1020702

Figure 4.

Figure 4

RT-PCR of S2 cell, Drosophila embryo, larva, and adult RNA. (A) 1.5% agarose/TBE gel of RT-PCR reactions for embryo RNA. The primer locations are indicated. Primers D and E are to the Drosophila GAPDH sequence; (lane 4) the cDNA− reaction; (lane 5) 1-kb DNA ladder. Similar results were seen for larvae, adult fly, and S2 cell RNA. (B) Shows 1.5% agarose/TBE gel of PCR reactions performed on the RT-PCR reactions of primer pair A/B; (lane 1) 1-kb DNA ladder; (lane 2) S2 cell; (lane 3) larva; (lane 4) embryo; (lane 5) adult; (lane 6) 100-bp DNA ladder. The arrows indicate the bands that were gel-purified and directly sequenced.