Figure 9.
RNA interference of stat92E activity in embryos. Cuticle preparations of embryos. (A) Wild-type embryo (buffer injection; 105 of 105), with abdominal segments 1, 3, and 6 labeled. (B) Injection of 5 μM ds-Exon3/4 RNA. The abdominal segments 4 and 5 are missing or fused (7 of 103). This phenotype is similar to paternally rescued hopscotch, stat92E, and domeless null embryos. (C) Injection of 5 μM ds-Exon2 RNA. Some embryos had the phenotype involving the abdominal segments 4 and 5 (16 of 153), whereas some had additional more severe segmentation defects involving the head and tail region (12 of 153). This phenotype is similar to parternally unrescued hopscotch, stat92E, and domeless null embryos. The images were scaled identically. Anterior is to the left.