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. 2002 Sep 15;16(18):2345–2349. doi: 10.1101/gad.996302

Figure 1.

Figure 1

lin-48 expression and function in C. elegans and C. briggsae. (a) Nomarski DIC micrograph of wild-type L3 C. elegans animal showing the excretory system. (b) Epi-fluorescent micrograph of animal in a, showing expression of Ce-lin-48∷gfp. The location of the excretory cell and excretory duct cell are indicated by an asterisk and an arrow. The excretory duct is indicated by an arrowhead. The excretory duct forms more posterior in wild-type C. elegans (c) than in C. elegans lin-48 mutants (d) or C. briggsae (e). ces-2 mutant animals exhibit the same excretory duct cell morphological phenotype as lin-48(sa469) mutants. Bracket shows distance between the duct and the base of the posterior pharynx. (f) lin-48 mutant males develop abnormal ectopic spicule cells anterior to the normal spicule cells. (g) lin-48 mutant animal rescued by Cb-lin-48 genomic clone, resulting in development of normal spicule cells. Arrows indicate cells expressing spicule cuticle. Scale bars, 10μm. (Left) anterior; (top) dorsal.