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. 2002 Sep 15;16(18):2365–2378. doi: 10.1101/gad.1013302

Figure 4.

Figure 4

Shh is required for the specification and delamination of cvg precursors. Transverse sections through the anterior portion of the otic vesicles of wild-type (a,c,e,g) and Shh−/− (b,d,f,h) embryos. NeuroD (a,b) and Ngn1 (c,d) expression is downregulated within cvg precursors of Shh−/− embryos at 9.5 dpc. NeuroD (e,f) and Fgf10 (g,h) expression is reduced within the cvg and geniculate ganglia of Shh−/− embryos at 10.5 dpc. Red lines in e and f highlight the protracted expression of NeuroD in the mutant vs. wild-type otic epithelium. Adjacent sections probed with Lfng (i,l), Bmp4 (j,m), and Myo XV (k,n) expression from wild-type and Shh−/− inner ears at 15.5 dpc. Arrows point to the positive Lfng and Myo XV domains that are negative for Bmp4. The level of the sections is shown in each representative diagram. ca, crista ampullaris; gg, geniculate ganglia; lc, lateral crista; mu, macula utriculi; pc, posterior crista. Bar, 100 μm.