Table 3.

Power calculations estimating the sample sizes required to detect a 10, 25, 33, 50, 75 and 100% shift in the response, as percentage change (i) at 120 min, (ii) AUC(0, 120 min) and (iii) AUC(90, 120 min); for each dose with a power of 90% and 80% and significance accepted at 5%.

(i)
		FBF ratio		FBF infused arm
ET-1 (pmol min−1)	% shift (120 min)	90% power	80% power	90% power	80% power
2.5	10	132	99	389	293
	25	22	16	63	47
	33	13	10	36	27
	50	6	≥ 5	16	12
	75	≥ 5	≥ 5	7	6
	100	≥ 5	≥ 5	≥ 5	≥ 5
10	10	38	29	122	92
	25	6	≥ 5	20	15
	33	≥ 5	≥ 5	12	9
	50	≥ 5	≥ 5	≥ 5	≥ 5
	75	≥ 5	≥ 5	≥ 5	≥ 5
	100	≥ 5	≥ 5	≥ 5	≥ 5

(ii)
		FBF ratio		FBF infused arm
ET-1 (pmol min−1)	% shiftAUC (0, 120 min)	90% power	80% power	90% power	80% power
2.5	10	180	136	1093	823
	25	29	22	175	132
	33	17	13	101	76
	50	8	6	44	33
	75	≥ 5	≥ 5	20	15
	100	≥ 5	≥ 5	11	9
10	10	33	25	144	108
	25	6	≥ 5	23	18
	33	≥ 5	≥ 5	14	10
	50	≥ 5	≥ 5	6	≥ 5
	75	≥ 5	≥ 5	≥ 5	≥ 5
	100	≥ 5	≥ 5	≥ 5	≥ 5

(iii)
		FBF ratio		FBF infused arm
ET-1 (pmol min−1)	% shiftAUC (90, 120 min)	90% power	80% power	90% power	80% power
2.5	10	83	63	368	277
	25	13	10	59	45
	33	8	6	34	26
	50	≥ 5	≥ 5	15	12
	75	≥ 5	≥ 5	7	≥ 5
	100	≥ 5	≥ 5	≥ 5	≥ 5
10	10	50	38	130	98
	25	8	6	21	16
	33	≥ 5	≥ 5	12	9
	50	≥ 5	≥ 5	6	≥ 5
	75	≥ 5	≥ 5	≥ 5	≥ 5
	100	≥ 5	≥ 5	≥ 5	≥ 5