Table 4.

Power calculations estimating the sample sizes required to detect a 25 and 50% shift in the response, as percentage change at 60 min, 90 min and 120 min and percentage change in AUC(0, 60 min), (30, 60 min), (0, 90 min), (60, 90 min), (0, 120 min) and (90, 120 min), for each dose with a power of 90% and significance accepted at 5%.

ET-1(pmol min−1)	FBF method	% shift	AUC (0, 60 min)	AUC (30, 60 min)	60 min	AUC (0, 90 min)	AUC (60, 90 min)	90 min	AUC (0, 120 min)	AUC (90, 120 min)	120 min
2.5	Ratio	25	116	59	30	52	22	20	29	13	22
		50	29	15	8	13	6	≥ 5	8	≥ 5	6
	Infused arm	25	726	369	165	296	92	165	175	59	6
		50	182	93	42	74	23	42	44	15	≥ 5
10	Ratio	25	42	15	≥ 5	13	8	12	6	8	6
		50	11	≥ 5	≥ 5	≥ 5	≥ 5	≥ 5	≥ 5	≥ 5	≥ 5
	Infused arm	25	59	31	27	34	22	27	23	21	15
		50	15	8	7	9	6	7	6	6	≥ 5