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. 2002 Nov;54(5):504–510. [Article in Spanish] doi: 10.1046/j.1365-2125.2002.01673.x

Table 1.

PCR primers and hybridization probes for allelic discrimination and sequencing of CYP1A2*1F. The bold figures show the mutation site.

Primer Sequences
TaqMan probe – TET 5′-CTC TGT GGG CCC AGG ACG CAT-3′
TaqMan probe – FAM 5′-TC TGT GGG CAC AGG ACG CAT GG-3′
TaqMan Forward primer 5′-TTT CCA GCT CTC AGA TTC TGT GAT-3′
TaqMan Reverse primer 5′-GGA TAC CAG AAA GAC TAA GCT CCA TC-3′
Forward primer* 5′-TTC CCC ATT TTG GAG TGG TC-3′
Reverse primer* 5′-CCG AGA AGG GAA CAG ACT GG-3′
*

Primers used for sequencing.