Figure 1.

Propagation and toxicity of PV1(RIPO) in malignant glioma tissue culture cell lines. (A) One-step growth curves of PV1(RIPO) were obtained after synchronized infection of monolayer cultures at a multiplicity of infection of 10 according to previous reports (10). Glioma cell lines tested included SF188 (open square; glioblastoma multiforme), SF295 (closed square; glioblastoma multiforme), SF767 (open triangle; anaplastic astrocytoma), SF763 (closed triangle; anaplastic astrocytoma), HTB-14 (open circle; anaplastic astrocytoma), HTB-15 (closed circle; anaplastic astrocytoma), DU54 (open diamond; glioblastoma multiforme), and DU65 (closed triangle; glioblastoma multiforme). (B) Synchronized infection of primary cultured glioblastoma multiforme cells. Cells were photographed before (a) and 12 h after (b) infection with PV1(RIPO) at a multiplicity of infection of 10.