Figure 1.

c-Myc is expressed in endothelial cells of the embryonic E8.5 yolk sac membrane. Endothelial cells were detected by immunofluorescence analyses with an antibody specific for PECAM-1 (red stain), which is expressed on the cell surface of endothelial cells. c-Myc expression was detected with a mouse-specific c-Myc antibody (green stain) and was largely confined to nuclei. Overlay shows that c-Myc-expressing cells (with green nuclei) also expressed PECAM-1. For clarity, the boxed areas in the top panels were magnified and are shown below.