Immunoelectron microscopy for SgIGSF in olfactory epithelia 0 day (A, B) and 11 days (C, D) after olfactory nerve transection. (A) Intense immunoreactivity is localized to cell surface in apical portions of the epithelium. Weaker immunoreactivity is recognized on the cell surface in other portions. S, sustentacular cells; Om, mature olfactory cells. (B) At higher magnification of apical epithelial portions, the membrane of the dendrites (D) of mature olfactory cells and the opposing membrane of sustentacular cells (S) are intensely immunostained. Membrane portions at the base of the olfactory vesicle (OV) are immunonegative. (C) Intense immunoreactivity is localized to the middle portions of the epithelium where immature olfactory cells (Oi) exist, whereas it is not recognized in the apical portions of the epithelium. S, sustentacular cells. (D) At higher magnification of middle epithelial portions, the surface of immature olfactory cells (Oi) and the opposing membrane of sustentacular cells (S) are intensely immunostained. Bars=5 µm (A, C) and 1 µm (B, D).