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. 2002 Oct 1;16(19):2479–2484. doi: 10.1101/gad.232902

Figure 2.

Figure 2

Distribution of active origins in the rDNA cluster of HU-arrested cells. Wild-type cells (E1000) were released from α-factor into S phase and exposed for 90 min to 0.2 M HU and 0.4 mg/mL BrdU. BrdU-labeled rDNA fibers were analyzed by DMC, as described in Figure 1. (A) Size distribution of BrdU tracks (b) and gaps (a) in single-copy sequences of the yeast genome. A total of 12.3 Mb of DNA fibers devoid of rDNA signals were analyzed. To allow comparison with rDNA, where replication is monodirectional, the distance covered by only one fork is represented. (B) BrdU tracks (b) and gaps (a) in rDNA fibers. A set of representative fibers is shown in Supplementary Figure 2 (see Supplementary Material at http://www.genesdev.org). (C) Example of long rDNA fiber (830 kb) showing very large gaps (>100 kb). (D) Junction between the rDNA cluster and flanking sequences on Chromosome XII. Here, digestion with BamHI and XhoI was omitted. (Red) FISH probes; (green) BrdU. Bar, 50 kb.