Table 2.
Volume changes for IHF binding to de novo synthetic sites
| Site | ΔVspec (ml mol−1) | ΔGspec (kcal mol–1) |  |
No. of expts | Def. energy (kcal mol−1) |
|---|---|---|---|---|---|
| A.2 | 112 ± 8.0 | −13.8 ± 0.25 | 1.60 | 4 | 87 |
| A.3 | 131 ± 9.3 | −12.3 ± 0.21 | 1.05 | 3 | 92 |
| A.6 | 154 ± 8.0 | −13.2 ± 0.15 | 1.64 | 5 | 142 |
Note: Results from pressure dissociation of binary complexes formed by sequence-specific binding of IHF to de novo sequences designed to vary the DNA deformation energy in the complex. Sequences contain the complete complement of consensus sequence elements as described in the text; the remaining sequence varies to generate the widest possible range of deformation energy. Values of the volume change (ΔVspec) free energy change for specific binding (ΔGspec) were obtained by global analysis of pressure-dissociation curves obtained with varying concentrations of IHF ranging from 9 to 50 nM and of DNA oligonucleotide ranging from 3 to 30 nM, as described in the text.