Table 3.

Salt dependence of IHF binding to de novo synthetic sites

Site	ΔVspeca (ml mol−1)	[NaCl] (mM)	ΔGspec (kcal mol−1)
A.2	112	100	−13.6 ± 0.08	1.06
		200	−12.5 ± 0.05
		400	−9.6 ± 0.13
A.3	131 ± 9.3	50	−13.0 ± 0.25	1.05
		100	−12.3 ± 0.21
		200	−9.8 ± 0.09
A.6	154 ± 8.0	50	−13.7 ± 0.22	1.00
		100	−13.2 ± 0.19
		200	−11.4 ± 0.15

Note: Values of the volume change (ΔV_spec) and free energy change for specific binding (ΔG_spec) were obtained by global analysis of pressure perturbation curves obtained at varying NaCl concentration using 50 nM IHF and 10 nM DNA oligonucleotide, as described in the text.

^aΔV_spec was held constant vs. NaCl concentrations for each oligonucleotide as discussed in the text. The value was poorly resolved in the analysis of oligonucleotide A.2, and so was held fixed at the value obtained from analysis of the data at 100 mM NaCl in Table 1.