Table 2.
Heterologous complementation of B. subtilis RNase P mutant strain SSB318 by S. aureus (type B) rnpB alleles
| rnpB variants in pHY300 | + IPTG | −IPTG | Aldose |
|---|---|---|---|
| S. aureus PBs rnpB rnpBwt | + + + | + + + | None |
| S. aureus PBs rnpB rnpBC238 | + + + | + | None |
| S. aureus PBs rnpB rnpBC239 | + + + | + | None |
| S. aureus PBs rnpB rnpBwt + Pxyl rnpA | + + + | + + + | Xylose |
| S. aureus PBs rnpB rnpBC238 + Pxyl rnpA | + + + | + + + | Xylose |
| S. aureus PBs rnpB rnpBC239 + Pxyl rnpA | + + + | + + + | Xylose |
Growth of mutant strain SSB318 transformed with wild-type S. aureus (rnpBwt) and respective mutant alleles on plasmid pHY300; promoter types: PBs rnpB, native B. subtilis rnpB promoter; Pxyl, inducible xylose promoter; B. subtilis rnpA was overexpressed in parallel from the same plasmid. Cell growth was analyzed on LB plates in the presence (1 mM) or absence of IPTG; the aldose xylose was added to 2% (w/v) for pHY300 derivatives encoding B. subtilis rnpA.
+ + +: growth with equal numbers of colonies on the corresponding + and − IPTG plates; +: retarded cell growth, but equal numbers of colonies on + and − IPTG plates