. 2007 Mar 13;35(6):2060–2073. doi: 10.1093/nar/gkm005

Table 5.

Cleavage rates for the B. subtilis wild-type RNase P holoenzyme acting on substrates with 3′-end variations at low magnesium concentrations

ptRNA^Gly	Enzyme concentration	[Mg²⁺]	k_obs	k_rel
U73	10 nM P RNA/ 37 nM RnpA	4.5	4.3 ± 0.08	0.36
U73UAAAUA	10 nM P RNA/ 37 nM RnpA	4.5	3.3 ± 0.05	0.28
U73CCAAUA	10 nM P RNA/ 37 nM RnpA	4.5	11.8 ± 0.15	1.0
U73	10 nM P RNA/ 37 nM RnpA	2.0	1.5 ± 0.01	0.21
U73UAAAUA	10 nM P RNA/ 37 nM RnpA	2.0	1.4 ± 0.01	0.2
U73CCAAUA	10 nM P RNA/ 37 nM RnpA	2.0	7.0 ± 0.04	1.0
U73	50 nM P RNA/ 185 nM RnpA	2.0	9.5 ± 0.08	0.19
U73UAAAUA	50 nM P RNA/ 185 nM RnpA	2.0	6.8 ± 0.03	0.13
U73CCAAUA	50 nM P RNA/ 185 nM RnpA	2.0	49.9 ± 0.43	1.0

For assay conditions, see legend to Table 4; k_rel is defined as the ratio of k_obs obtained with the mutant versus canonical (U73CCAAUA) substrate under the respective conditions; values are based on at least four independent experiments.