Figure 1.

Single microgene-based method. A single microgene block has motif A (red) and motif B (cyan) embedded in different reading frames. The microgene (shown by the double helix) is polymerized in tandem using a microgene polymerization reaction (MPR) in which two overlapping MPR primer pairs having a 3′ mismatched base (shown by red letters with dots) are used. During MPR, insertion and/or deletion mutations randomly occur at junctions between microgene blocks (shown by wavy lines labeled ‘FS’) causing the reading frame to randomly shift, leading to combinatorial polymerization of motifs A and B.