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. 2007 Feb 22;35(6):e45. doi: 10.1093/nar/gkm047

Figure 1.

Figure 1.

Vector derivations and maps. Derivation of the pOPIN vectors from pTriEx2. PCR fragments were prepared as described in the Materials and methods section and either ligated into the pTriEx2 vector or inserted by In-Fusion™. In cases where the pOPIN vector is not directly derivatized from pTriEx2, the intermediate vector is also shown. Features of the pTriEx2 vector retained in the pOPIN vector suite are: T7/lacO promoter/operator and terminator for inducible expression in E. coli harbouring the λ (DE3) prophage, CMV Enhancer/Chicken β-actin promoter and rabbit β-globin polyA site for efficient expression in mammalian hosts, p10 baculoviral promoter and 5′ UTR/ORF603 and ORF 1629 for efficient expression from/recombination into baculovirus respectively. The high-copy pUC origin of replication and β-lactamase (Ampicillin resistance marker) gene allow high-copy production of the vector in E. coli.